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Journal of Lipid Research, Vol 19, 742-746, Copyright © 1978 by Lipid Research, Inc.

ARTICLES

Resolution of desmosterol, cholesterol, and other sterol intermediates by reverse-phase high-pressure liquid chromatography

E Hansbury and TJ Scallen

A highly efficient technique has been developed for the resolution of several sterols that are intermediates in the biosynthesis of cholesterol and that differ only by one carbon-carbon double bond or by one methyl group. The technique described utilizes reverse-phase high- pressue liquid chromatography on a micronBondapak-C18 column with acetonitrile as eluting solvent. This procedure is capable of measuring the enzymatic conversion of desmosterol to cholesterol. This chromatographic separation can be conducted by reverse-phase high- pressure liquid chromatography in approximately 10 min, whereas other procedures can require several days.
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