J. Lipid Res. Acyl Labeled PIP's available August 1, 2008
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Journal of Lipid Research, Vol 19, 774-777, Copyright © 1978 by Lipid Research, Inc.

ARTICLES

Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography

BR Krause and AD Hartman

A reliable method for the separation of free and esterified cholesterol in adipocyte extracts is described. The procedure uses Sephadex LH-20, a lipophilic dextran gel, with a solvent system of chloroform-hexane 55:45 (v/v). Interference by excess triglyceride, such as that encountered in adipocyte total lipid extracts, was not observed, and overall recovery of both sterols exceeded 98%.
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Copyright © 1978 by the American Society for Biochemistry and Molecular Biology.