Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography

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Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography

BR Krause and AD Hartman

A reliable method for the separation of free and esterified cholesterol in adipocyte extracts is described. The procedure uses Sephadex LH-20, a lipophilic dextran gel, with a solvent system of chloroform-hexane 55:45 (v/v). Interference by excess triglyceride, such as that encountered in adipocyte total lipid extracts, was not observed, and overall recovery of both sterols exceeded 98%.
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