J. Lipid Res. Acyl Labeled PIP's available August 1, 2008
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Journal of Lipid Research, Vol 19, 783-787, Copyright © 1978 by Lipid Research, Inc.

ARTICLES

Bile acids. OV. 2,2-Dimethoxypropane: an esterifying agent preferred to diazomethane for chenodeoxycholic acid

R Shaw and WH Elliott

Artifacts formed by methylation of chenodeoxycholic acid with diazomethane are identical to the synthetic 3alpha- and 7 alpha- monomethyl ethers of methyl chenodeoxycholate. The extent of formation of these artifacts with time has been studied. No detectable artifacts were found with 2,2-dimethoxypropane as the methylating agent.
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