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Journal of Lipid Research, Vol. 19, 836-840, September 1978
Copyright © 1978 by Lipid Research, Inc.

Sterol synthesis and CO2 production from mevalonate in calves

James R. Linder and Donald C. Beitz

Department of Animal Science, Iowa State University of Science and Technology, Ames, IA 50011

Nonruminating male Holstein calves were fed a reconstituted milk containing 11.7% nonfat-dried-milk solids and 3.5% beef tallow. Calves were slaughtered at 17 weeks of age. Samples of perirenal adipose tissue, liver, muscle, small intestine, kidney cortex, and kidney medulla were assayed in vitro for sterol synthesis and production of 14CO2 from [2-14C]mevalonate. Of the tissues tested, adipose tissue and kidney medulla produced nonsaponified lipids at the greatest rates; kidney cortex and liver at half the rate of adipose tissue; muscle and jejunum at one-eighth the rate of adipose tissue; and ileum at a negligible rate. The amount of 14C in squalene, lanosterol, and cholesterol of the nonsaponified lipids of each tissue was determined by thin-layer chromatography. Proportions of 14C in cholesterol to 14C in total nonsaponified lipids ranged from 30% to 59%; squalene, from 5% to 27%; and lanosterol, from 11% to 59% of the total nonsaponified lipids present. The rate of CO2 production by the "trans-methylglutaconate shunt of mevalonate metabolism" was determined. Kidney cortex displayed the greatest shunt activity, producing 15-80-fold more CO2 than any other tissue tested. Ileum, jejunum, skeletal muscle, and kidney medulla had similar shunt activities; liver sections had less shunt activity, and no shunt activity was detected in adipose tissue. These data reveal a shunt for mevalonate utilization that does not lead to sterols and also show that the kidney is important in the sterol and nonsterol metabolism of mevalonate.

Supplementary key words cholesterol synthesis • trans-methyl-glutaconate shunt pathway

Submitted on September 27, 1977
Accepted on March 15, 1978


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