Journal of Lipid Research, Vol 19, 922-924, Copyright © 1978 by Lipid Research, Inc.
The preparative isolation of lecithin
NS Radin
Lecithin can be prepared on a relatively large scale, free of colored
impurities, by a simple two-column procedure. Commercial crude egg lecithin
is partially purified by a single-step passage through an alumina column.
It is then purified by a two-step passage through a prepacked, commercial
silica gel column. The lecithin is prepared in solvent-free form for
weighing by lyophilization from cyclohexane. Toxic solvents (chloroform and
methanol) are avoided by the use of ethanol, isopropanol, hexane, and
water. The elutions are easily monitored by a flow cell in an ordinary
spectrophotometer set at 215 nm. Study of the column parameters has made it
possible to use heavy loads with a relatively small column and minimal
solvent.
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