Journal of Lipid Research, Vol 19, 924-928, Copyright © 1978 by Lipid Research, Inc.

ARTICLES

Re-evaluation of the 3 alpha-hydroxysteroid dehydrogenase assay for total bile acids in bile

SD Turley and JM Dietschy

A review of the 3 alpha-hydroxysteroid dehydrogenase method for determining the concentration of total bile acids in bile is described. The optimum conditions for the assay were established with respect to pH, temperature, incubation time, amount of NAD+, and units of enzyme activity required to obtain complete oxidation of the substrate under fixed conditions. Furthermore, the effect of hydrazine hydrate, methanol, and bile volume on the reaction was examined. It was also established that the bile acid concentration in bile samples with a high molar percentage of cholesterol would be overestimated if 3 beta- hydroxysteroid dehydrogenase were present with the 3 alpha-enzyme.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. Freudenberg, A. L. Broderick, B. B. Yu, M. R. Leonard, J. N. Glickman, and M. C. Carey Pathophysiological basis of liver disease in cystic fibrosis employing a {Delta}F508 mouse model Am J Physiol Gastrointest Liver Physiol, June 1, 2008; 294(6): G1411 - G1420. [Abstract] [Full Text] [PDF]

				D. Jung, T. Inagaki, R. D. Gerard, P. A. Dawson, S. A. Kliewer, D. J. Mangelsdorf, and A. Moschetta FXR agonists and FGF15 reduce fecal bile acid excretion in a mouse model of bile acid malabsorption J. Lipid Res., December 1, 2007; 48(12): 2693 - 2700. [Abstract] [Full Text] [PDF]

				D. Alvaro, G. Macarri, M. G. Mancino, M. Marzioni, M. Bragazzi, P. Onori, S. G. Corradini, P. Invernizzi, A. Franchitto, A. F. Attili, et al. Serum and Biliary Insulin-like Growth Factor I and Vascular Endothelial Growth Factor in Determining the Cause of Obstructive Cholestasis Ann Intern Med, October 2, 2007; 147(7): 451 - 459. [Abstract] [Full Text] [PDF]

				M. G. Jeschke, U. Bolder, D. H. Chung, R. Przkora, U. Mueller, J. C. Thompson, S. E. Wolf, and D. N. Herndon Gut Mucosal Homeostasis and Cellular Mediators after Severe Thermal Trauma and the Effect of Insulin-Like Growth Factor-I in Combination with Insulin-Like Growth Factor Binding Protein-3 Endocrinology, January 1, 2007; 148(1): 354 - 362. [Abstract] [Full Text] [PDF]

				M. T. Flowers, A. K. Groen, A. T. Oler, M. P. Keller, Y. Choi, K. L. Schueler, O. C. Richards, H. Lan, M. Miyazaki, F. Kuipers, et al. Cholestasis and hypercholesterolemia in SCD1-deficient mice fed a low-fat, high-carbohydrate diet J. Lipid Res., December 1, 2006; 47(12): 2668 - 2680. [Abstract] [Full Text] [PDF]

				K. J. van Erpecum, D. Q-H. Wang, A. Moschetta, D. Ferri, M. Svelto, P. Portincasa, J.-J. Hendrickx, M. Schipper, and G. Calamita Gallbladder histopathology during murine gallstone formation: relation to motility and concentrating function J. Lipid Res., January 1, 2006; 47(1): 32 - 41. [Abstract] [Full Text] [PDF]

				R. E. Temel, R. G. Lee, K. L. Kelley, M. A. Davis, R. Shah, J. K. Sawyer, M. D. Wilson, and L. L. Rudel Intestinal cholesterol absorption is substantially reduced in mice deficient in both ABCA1 and ACAT2 J. Lipid Res., November 1, 2005; 46(11): 2423 - 2431. [Abstract] [Full Text] [PDF]

				A. Moschetta, F. Xu, L. R. Hagey, G. P. van Berge-Henegouwen, K. J. van Erpecum, J. F. Brouwers, J. C. Cohen, M. Bierman, H. H. Hobbs, J. H. Steinbach, et al. A phylogenetic survey of biliary lipids in vertebrates, J. Lipid Res., October 1, 2005; 46(10): 2221 - 2232. [Abstract] [Full Text] [PDF]

				J. M. Martins, M. Riottot, M. C. de Abreu, A. M. Viegas-Crespo, M. J. Lanca, J. A. Almeida, J. B. Freire, and O. P. Bento Cholesterol-lowering effects of dietary blue lupin (Lupinus angustifolius L.) in intact and ileorectal anastomosed pigs J. Lipid Res., July 1, 2005; 46(7): 1539 - 1547. [Abstract] [Full Text] [PDF]

				D. Weihs, J. Schmidt, I. Goldiner, D. Danino, M. Rubin, Y. Talmon, and F. M. Konikoff Biliary cholesterol crystallization characterized by single-crystal cryogenic electron diffraction J. Lipid Res., May 1, 2005; 46(5): 942 - 948. [Abstract] [Full Text] [PDF]

				L. Yu, S. Gupta, F. Xu, A. D. B. Liverman, A. Moschetta, D. J. Mangelsdorf, J. J. Repa, H. H. Hobbs, and J. C. Cohen Expression of ABCG5 and ABCG8 Is Required for Regulation of Biliary Cholesterol Secretion J. Biol. Chem., March 11, 2005; 280(10): 8742 - 8747. [Abstract] [Full Text] [PDF]

				M Pizarro, N Balasubramaniyan, N Solis, A Solar, I Duarte, J F Miquel, F J Suchy, M Trauner, L Accatino, M Ananthanarayanan, et al. Bile secretory function in the obese Zucker rat: evidence of cholestasis and altered canalicular transport function Gut, December 1, 2004; 53(12): 1837 - 1843. [Abstract] [Full Text] [PDF]

				J. M. Martins, M. Riottot, M. C. de Abreu, M. J. Lanca, A. M. Viegas-Crespo, J. A. Almeida, J. B. Freire, and O. P. Bento Dietary Raw Peas (Pisum sativum L.) Reduce Plasma Total and LDL Cholesterol and Hepatic Esterified Cholesterol in Intact and Ileorectal Anastomosed Pigs Fed Cholesterol-Rich Diets J. Nutr., December 1, 2004; 134(12): 3305 - 3312. [Abstract] [Full Text] [PDF]

				G H Koek, R Vos, P Flamen, D Sifrim, F Lammert, B Vanbilloen, J Janssens, and J Tack Oesophageal clearance of acid and bile: a combined radionuclide, pH, and Bilitec study Gut, January 1, 2004; 53(1): 21 - 26. [Abstract] [Full Text] [PDF]

				G A Clarke, G Bouchard, B Paigen, and M C Carey Cholesterol synthesis inhibition distal to squalene upregulates biliary phospholipid secretion and counteracts cholelithiasis in the genetically prone C57L/J mouse Gut, January 1, 2004; 53(1): 136 - 142. [Abstract] [Full Text] [PDF]

				M.-Y. Akoume, S. Perwaiz, I. M. Yousef, and G. L. Plaa Synergistic Role of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase and Cholesterol 7{alpha}-Hydroxylase in the Pathogenesis of Manganese-Bilirubin-Induced Cholestasis in Rats Toxicol. Sci., June 1, 2003; 73(2): 378 - 385. [Abstract] [Full Text] [PDF]

				N. Mendez-Sanchez, V. Gonzalez, A. C. King-Martinez, H. Sanchez, and M. Uribe Plasma Leptin and the Cholesterol Saturation of Bile Are Correlated in Obese Women after Weight Loss J. Nutr., August 1, 2002; 132(8): 2195 - 2198. [Abstract] [Full Text] [PDF]

				A. Moschetta, P. M. Frederik, P. Portincasa, G. P. vanBerge-Henegouwen, and K. J. van Erpecum Incorporation of cholesterol in sphingomyelin- phosphatidylcholine vesicles has profound effects on detergent-induced phase transitions J. Lipid Res., July 1, 2002; 43(7): 1046 - 1053. [Abstract] [Full Text] [PDF]

				N A M van Ooteghem, A Moschetta, J F Rehfeld, M Samsom, K J van Erpecum, and G P van Berge-Henegouwen Intraduodenal conjugated bile salts exert negative feedback control on gall bladder emptying in the fasting state without affecting cholecystokinin release or antroduodenal motility Gut, May 1, 2002; 50(5): 669 - 674. [Abstract] [Full Text] [PDF]

				P. Portincasa, N. G. Venneman, A. Moschetta, A. van den Berg, G. Palasciano, G. P. vanBerge-Henegouwen, and K. J. van Erpecum Quantitation of cholesterol crystallization from supersaturated model bile J. Lipid Res., April 1, 2002; 43(4): 604 - 610. [Abstract] [Full Text] [PDF]

				A.-M. Montet, L. Oliva, F. Beauge, and J.-C. Montet BILE SALTS MODULATE CHRONIC ETHANOL-INDUCED HEPATOTOXICITY Alcohol Alcohol., January 1, 2002; 37(1): 25 - 29. [Abstract] [Full Text] [PDF]

				M. Schwarz, D. L. Davis, B. R. Vick, and D. W. Russell Genetic analysis of intestinal cholesterol absorption in inbred mice J. Lipid Res., November 1, 2001; 42(11): 1801 - 1811. [Abstract] [Full Text] [PDF]

				N. Mendez-Sanchez, V. Gonzalez, P. Aguayo, J. M. Sanchez, M. A. Tanimoto, J. Elizondo, and M. Uribe Fish Oil (n-3) Polyunsaturated Fatty Acids Beneficially Affect Biliary Cholesterol Nucleation Time in Obese Women Losing Weight J. Nutr., September 1, 2001; 131(9): 2300 - 2303. [Abstract] [Full Text] [PDF]

				A. Moschetta, G. P. vanBerge-Henegouwen, P. Portincasa, G. Palasciano, and K. J. van Erpecum Cholesterol crystallization in model biles: effects of bile salt and phospholipid species composition J. Lipid Res., August 1, 2001; 42(8): 1273 - 1281. [Abstract] [Full Text] [PDF]

				A. Adam, M.-A. Levrat-Verny, H. W. Lopez, M. Leuillet, C. Demigné, and C. Rémésy Whole Wheat and Triticale Flours with Differing Viscosities Stimulate Cecal Fermentations and Lower Plasma and Hepatic Lipids in Rats J. Nutr., June 1, 2001; 131(6): 1770 - 1776. [Abstract] [Full Text]

				H. W. Lopez, M.-A. Levrat-Verny, C. Coudray, C. Besson, V. Krespine, A. Messager, C. Demigné, and C. Rémésy Class 2 Resistant Starches Lower Plasma and Liver Lipids and Improve Mineral Retention in Rats J. Nutr., April 1, 2001; 131(4): 1283 - 1289. [Abstract] [Full Text]

				P. Mardones, V. Quiñones, L. Amigo, M. Moreno, J. F. Miquel, M. Schwarz, H. E. Miettinen, B. Trigatti, M. Krieger, S. VanPatten, et al. Hepatic cholesterol and bile acid metabolism and intestinal cholesterol absorption in scavenger receptor class B type I-deficient mice J. Lipid Res., February 1, 2001; 42(2): 170 - 180. [Abstract] [Full Text]

				J. Férézou, M. Combettes-Souverain, M. Souidi, J. L. Smith, N. Boehler, F. Milliat, E. Eckhardt, G. Blanchard, M. Riottot, C. Sérougne, et al. Cholesterol, bile acid, and lipoprotein metabolism in two strains of hamster, one resistant, the other sensitive (LPN) to sucrose-induced cholelithiasis J. Lipid Res., December 1, 2000; 41(12): 2042 - 2054. [Abstract] [Full Text]

				A. Moschetta, G. P. vanBerge-Henegouwen, P. Portincasa, G. Palasciano, A. K. Groen, and K. J. van Erpecum Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts J. Lipid Res., June 1, 2000; 41(6): 916 - 924. [Abstract] [Full Text]

				K. A. Greaves, M. D. Wilson, L. L. Rudel, J. K. Williams, and J. D. Wagner Consumption of Soy Protein Reduces Cholesterol Absorption Compared to Casein Protein Alone or Supplemented with an Isoflavone Extract or Conjugated Equine Estrogen in Ovariectomized Cynomolgus Monkeys J. Nutr., April 1, 2000; 130(4): 820 - 826. [Abstract] [Full Text]

				E. R. M. Eckhardt, A. Moschetta, W. Renooij, S. S. Goerdayal, G. P. van Berge-Henegouwen, and K. J. van Erpecum Asymmetric distribution of phosphatidylcholine and sphingomyelin between micellar and vesicular phases: potential implications for canalicular bile formation J. Lipid Res., November 1, 1999; 40(11): 2022 - 2033. [Abstract] [Full Text]

				A. van Helvoort, A. de Brouwer, R. Ottenhoff, J. F. H. M. Brouwers, J. Wijnholds, J. H. Beijnen, A. Rijneveld, T. van der Poll, M. A. van der Valk, D. Majoor, et al. Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces PNAS, September 28, 1999; 96(20): 11501 - 11506. [Abstract] [Full Text] [PDF]

				N. Boehler, M. Riottot, J. Férézou, M. Souidi, F. Milliat, C. Sérougne, J. L. Smith, and C. Lutton Antilithiasic effect of ß-cyclodextrin in LPN hamster: comparison with cholestyramine J. Lipid Res., April 1, 1999; 40(4): 726 - 734. [Abstract] [Full Text]

				D. Q-H. Wang, D. E. Cohen, F. Lammert, and M. C. Carey No pathophysiologic relationship of soluble biliary proteins to cholesterol crystallization in human bile J. Lipid Res., March 1, 1999; 40(3): 415 - 425. [Abstract] [Full Text]

				E. R. M. Eckhardt, B. J. M. van de Heijning, K. J. van Erpecum, W. Renooij, and G. P. VanBerge-Henegouwen Quantitation of cholesterol-carrying particles in human gallbladder bile J. Lipid Res., March 1, 1998; 39(3): 594 - 603. [Abstract] [Full Text]

				B C Sharma, D K Agarwal, S S Baijal, T S Negi, G Choudhuri, and V A Saraswat Effect of endoscopic sphincterotomy on gall bladder bile lithogenicity and motility Gut, February 1, 1998; 42(2): 288 - 292. [Abstract] [Full Text] [PDF]