J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol 19, 1032-1037, Copyright © 1978 by Lipid Research, Inc.

ARTICLES

Purification of pancreatic lipase via its affinity for bile salts and apolar surfaces

WE Momsen and HL Brockman

An improved method for the purification of pancreatic lipase in milligram amounts is described. Only one batch step and one chromatographic step are required to produce a lipase-colipase complex and a lipase largely free of colipase. Both species are lipid free; physical and kinetic data indicate that the predominant lipase species is lipase B.
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Copyright © 1978 by the American Society for Biochemistry and Molecular Biology.