| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 19, 1063-1067, Copyright © 1978 by Lipid Research, Inc.
ARTICLES |
J Schacht
The binding of polyphosphoinositides (phosphatidylinositol phosphate and phosphatidylinositol bisphosphate) to the antibiotic neomycin is utilized for the purification of these lipids. Neomycin is reductively coupled to reactive glass beads (Glycophase-CPG) and serves as the stationary phase in column chromatography. A total lipid extract is prepared from tissues with chloroform-methanol-KC1 or chloroform- methanol-HC1 and washed once with acidified methanol-water. After the addition of an equal volume of methanolic 200 mM ammonium acetate, the extract is directly applied to the column. All lipids but the polyphosphoinositides are removed from the column by rinsing with 150 mM ammonium acetate in chloroform-methanol-water. Increasing the salt concentration to 600 mM elutes phosphatidylinositol phosphate. While further increases in ionic strength are not sufficient for a quantitative removal of phosphatidylinositol bisphosphate, the lipid is completely eluted by the addition of either ammonia or HC1 to the solvent. The column can be recycled and used repeatedly.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Guillou, C. Lecureuil, K. E. Anderson, S. Suire, G. J. Ferguson, C. D. Ellson, A. Gray, N. Divecha, P. T. Hawkins, and L. R. Stephens Use of the GRP1 PH domain as a tool to measure the relative levels of PtdIns(3,4,5)P3 through a protein-lipid overlay approach J. Lipid Res., March 1, 2007; 48(3): 726 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. V. Hammond, S. K. Dove, A. Nicol, J. A. Pinxteren, D. Zicha, and G. Schiavo Elimination of plasma membrane phosphatidylinositol (4,5)-bisphosphate is required for exocytosis from mast cells J. Cell Sci., May 15, 2006; 119(10): 2084 - 2094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Nikolaeva, B. Mukherjee, and P. K. Stys Na+-Dependent Sources of Intra-Axonal Ca2+ Release in Rat Optic Nerve during In Vitro Chemical Ischemia J. Neurosci., October 26, 2005; 25(43): 9960 - 9967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Shimaya, K. S. Kovacina, and R. A. Roth On the Mechanism for Neomycin Reversal of Wortmannin Inhibition of Insulin Stimulation of Glucose Uptake J. Biol. Chem., December 31, 2004; 279(53): 55277 - 55282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Fukami, M. Yoshida, T. Inoue, M. Kurokawa, R. A. Fissore, N. Yoshida, K. Mikoshiba, and T. Takenawa Phospholipase C{delta}4 is required for Ca2+ mobilization essential for acrosome reaction in sperm J. Cell Biol., April 14, 2003; 161(1): 79 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Mahnke-Zizelman and R. L. Sabina N-terminal Sequence and Distal Histidine Residues Are Responsible for pH-regulated Cytoplasmic Membrane Binding of Human AMP Deaminase Isoform E J. Biol. Chem., November 1, 2002; 277(45): 42654 - 42662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. HOLZ and D. AXELROD Localization of Phosphatidylinositol 4,5-P2 Important in Exocytosis and a Quantitative Analysis of Chromaffin Granule Motion Adjacent to the Plasma Membrane Ann. N.Y. Acad. Sci., October 1, 2002; 971(1): 232 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ruelland, C. Cantrel, M. Gawer, J.-C. Kader, and A. Zachowski Activation of Phospholipases C and D Is an Early Response to a Cold Exposure in Arabidopsis Suspension Cells Plant Physiology, October 1, 2002; 130(2): 999 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yonemura, T. Matsui, S. Tsukita, and S. Tsukita Rho-dependent and -independent activation mechanisms of ezrin/radixin/moesin proteins: an essential role for polyphosphoinositides in vivo J. Cell Sci., June 15, 2002; 115(12): 2569 - 2580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ozaki, D. B. DeWald, J. C. Shope, J. Chen, and G. D. Prestwich Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers PNAS, September 22, 2000; (2000) 210197897. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Mayer, D. Scheglmann, S. Dove, A. Glatz, W. Wickner, and A. Haas Phosphatidylinositol 4,5-Bisphosphate Regulates Two Steps of Homotypic Vacuole Fusion Mol. Biol. Cell, March 1, 2000; 11(3): 807 - 817. [Abstract] [Full Text]
|
|
|
|
|
|
T. Westergren, L. Ekblad, B. Jergil, and M. Sommarin Phosphatidylinositol 4-Kinase Associated with Spinach Plasma Membranes. Isolation and Characterization of Two Distinct Forms Plant Physiology, October 1, 1999; 121(2): 507 - 516. [Abstract] [Full Text]
|
|
|
|
|
|
L. S. Arneson, J. Kunz, R. A. Anderson, and L. M. Traub Coupled Inositide Phosphorylation and Phospholipase D Activation Initiates Clathrin-coat Assembly on Lysosomes J. Biol. Chem., June 18, 1999; 274(25): 17794 - 17805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Jones and M. Wessling-Resnick Inhibition of in Vitro Endosomal Vesicle Fusion Activity by Aminoglycoside Antibiotics J. Biol. Chem., September 25, 1998; 273(39): 25301 - 25309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Itoh, T. Ijuin, and T. Takenawa A Novel Phosphatidylinositol-5-phosphate 4-Kinase (Phosphatidylinositol-phosphate Kinase IIgamma ) Is Phosphorylated in the Endoplasmic Reticulum in Response to Mitogenic Signals J. Biol. Chem., August 7, 1998; 273(32): 20292 - 20299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Loyet, J. A. Kowalchyk, A. Chaudhary, J. Chen, G. D. Prestwich, and T. F. J. Martin Specific Binding of Phosphatidylinositol 4,5-Bisphosphate to Calcium-dependent Activator Protein for Secretion (CAPS), a Potential Phosphoinositide Effector Protein for Regulated Exocytosis J. Biol. Chem., April 3, 1998; 273(14): 8337 - 8343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Sankaran, J. Osterhout, D. Wu, and A. V. Smrcka Identification of a Structural Element in Phospholipase C beta 2 That Interacts with G Protein beta gamma Subunits J. Biol. Chem., March 20, 1998; 273(12): 7148 - 7154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Pappan, S. Zheng, and X. Wang Identification and Characterization of a Novel Plant Phospholipase D That Requires Polyphosphoinositides and Submicromolar Calcium for Activity in Arabidopsis J. Biol. Chem., March 14, 1997; 272(11): 7048 - 7054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Pappan, W. Qin, J. H. Dyer, L. Zheng, and X. Wang Molecular Cloning and Functional Analysis of Polyphosphoinositide-dependent Phospholipase D, PLDbeta , from Arabidopsis J. Biol. Chem., March 14, 1997; 272(11): 7055 - 7061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Romoser, R. Ball, and A. V. Smrcka Phospholipase C beta 2 Association with Phospholipid Interfaces Assessed by Fluorescence Resonance Energy Transfer. G PROTEIN beta &ggr; SUBUNIT-MEDIATED TRANSLOCATION IS NOT REQUIRED FOR ENZYME ACTIVATION J. Biol. Chem., October 11, 1996; 271(41): 25071 - 25078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fukami, N. Sawada, T. Endo, and T. Takenawa Identification of a Phosphatidylinositol 4,5-Bisphosphate-binding Site in Chicken Skeletal Muscle alpha-Actinin J. Biol. Chem., February 2, 1996; 271(5): 2646 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Liu, K. Fukami, H. Yu, and T. Takenawa A New Phospholipase C [IMAGE]4 Is Induced at S-phase of the Cell Cycle and Appears in the Nucleus J. Biol. Chem., January 5, 1996; 271(1): 355 - 360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Pertile, M. Liscovitch, V. Chalifa, and L. C. Cantley Phosphatidylinositol 4,5-Bisphosphate Synthesis Is Required for Activation of Phospholipase D in U937 Cells J. Biol. Chem., March 10, 1995; 270(10): 5130 - 5135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D Gupta, A Tartakoff, and E Tisdale Metabolic correction of defects in the lipid anchoring of Thy-1 in lymphoma mutants Science, December 9, 1988; 242(4884): 1446 - 1448. [Abstract] [PDF]
|
|
|
|
|
|
T. Itoh, H. Ishihara, Y. Shibasaki, Y. Oka, and T. Takenawa Autophosphorylation of Type I Phosphatidylinositol Phosphate Kinase Regulates Its Lipid Kinase Activity J. Biol. Chem., June 16, 2000; 275(25): 19389 - 19394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Holz, M. D. Hlubek, S. D. Sorensen, S. K. Fisher, T. Balla, S. Ozaki, G. D. Prestwich, E. L. Stuenkel, and M. A. Bittner A Pleckstrin Homology Domain Specific for Phosphatidylinositol 4,5-Bisphosphate (PtdIns-4,5-P2) and Fused to Green Fluorescent Protein Identifies Plasma Membrane PtdIns-4,5-P2 as Being Important in Exocytosis J. Biol. Chem., June 2, 2000; 275(23): 17878 - 17885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Park, T. Itoh, and T. Takenawa Phosphatidylinositol 4-Phosphate 5-Kinase Type I Is Regulated through Phosphorylation Response by Extracellular Stimuli J. Biol. Chem., February 9, 2001; 276(7): 4781 - 4787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ozaki, D. B. DeWald, J. C. Shope, J. Chen, and G. D. Prestwich Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers PNAS, October 10, 2000; 97(21): 11286 - 11291. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
