Journal of Lipid Research, Vol. 2, 37-44, January 1961
Copyright © 1961 by Lipid Research, Inc.

A new method for the analysis of component mono-, di-, and triglycerides

O. S. Privett and M. L. Blank

The Hormel Institute, University of Minnesota, Austin, Minnesota

A new micromethod is described for the determination of component mono-, di-, and triglycerides. The basic procedure involves ozonization of the double bonds and catalytic reduction of the ozonides followed by separation and quantification of the glyceryl residues by thinlayer chromatography. The potentialities of the method are demonstrated by the analysis of soybean oil and lard as well as standard mixtures of synthetic mono-, di-, and triglycerides. Procedures for the analysis of the four monoglyceride types, six of the seven possible diglyceride types, and four of the six possible triglyceride types are demonstrated.

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