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Journal of Lipid Research, Vol. 2, 45-50, January 1961
Copyright © 1961 by Lipid Research, Inc.

A fluorimetric micro glycerol method and its application to the determination of serum triglycerides

Dennis Mendelsohn and Arnold Antonis

Department of Pathology and Microbiology, Division of Chemical Pathology, University of the Witwatersrand, Johannesburg, South Africa; and Ernest Oppenheimer Heart Research Unit, South African Institute for Medical Research, Johannesburg, South Africa

A fluorimetric method has been developed for the estimation of glycerol in aqueous solution. It utilizes a series of reactions in which glycerol is heated with o-aminophenol in the presence of concentrated sulfuric acid and an oxidizing agent, to form 8-hydroxyquinoline which produces fluorescence on chelation with a divalent metal ion in alkaline solution. Experimental details are given for the estimation of serum triglycerides on phospholipid-free serum lipid extracts. The method can also be used for the estimation of phosphatide glycerol.

Submitted on April 18, 1960


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