Journal of Lipid Research, Vol. 2, 58-62, January 1961
Copyright © 1961 by Lipid Research, Inc.

The use of pancreatic lipase for determining the distribution of fatty acids in partial and complete glycerides

F. H. Mattson and R. A. Volpenhein

The Procter and Gamble Company, Miami Valley Laboratories, Cincinnati 39, Ohio

The method of digestion with pancreatic lipase for determining the position of fatty acids in triglycerides has been modified to increase its accurary and ease of applicability. Moreover, by acylation of partial glycerides with a "marker" fatty acid, the method can be used for determining the structure of mono- and diglycerides. Evidence is presented which demonstrates that acylation of partial glycerides with fatty acid chlorides can be carried out without causing rearrangement of the partial glycerides.

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