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Journal of Lipid Research, Vol. 2, 63-67, January 1961
Copyright © 1961 by Lipid Research, Inc.

Countercurrent distribution of soybean fatty acid methyl esters biosynthetically labeled with H3 and C14

H. J. Dutton , E. P. Jones , C. R. Scholfield , W. Chorney , and N. J. Scully

Northern Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois; and Argonne National Laboratory, Lemont, Illinois

Fatty acids randomly labeled with C14 and with H3 were prepared by culturing soybean plants (a) in the presence of C14O2, and (b) with H3OH in the nutrient medium. After maturation of the beans, extraction of the oils, and transesterification of the glycerides with methanol, the methyl esters were readily separated by countercurrent distribution to yield fractions of pure linoleate (7 g and 469 µc of C14-labeled esters), 97% linolenate, a 2:1 mixture of oleate and palmitate, 83% stearate, and concentrates of C20 and higher acid esters. Over-all recoveries of 90% may be expected from countercurrent distribution.

Submitted on May 4, 1960


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