Journal of Lipid Research, Vol. 2, 172-176, April 1961
Copyright © 1961 by Lipid Research, Inc.

The uptake of amino acids by lipids of pseudomonas aeruginosa

R. Silberman and W. L. Gaby

Department of Microbiology, Hahnemann Medical College and Hospital, Philadelphia 2, Pennsylvania

The lipids of intact Pseudomonas aeruginosa resting cells were extracted and the weights of the lipid fractions determined. The phospholipids were found to comprise a major portion (80%) of the ether-soluble lipids. Chromatograms of the phospholipid complex on silicic acid-impregnated paper revealed at least six fractions. Two-dimensional chromatograms of the aqueous acid hydrolyzate of the phospholipids indicated the presence of at least 13 ninhydrin positive compounds. There was a correlation between the uptake of dl-alanine-1-C¹⁴, dl-leucine-1-C¹⁴, and dl-phenylalanine-3-C¹⁴ by phospholipids of the resting cells and utilization of these amino acids by the cell as indicated by manometric studies.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?