J. Lipid Res.
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Journal of Lipid Research, Vol. 2, 203-207, July 1961
Copyright © 1961 by Lipid Research, Inc.

Gas chromatographic analysis of fatty acids from dialyzed lipoproteins

Alex V. Nichols , Carl S. Rehnborg , and Frank T. Lindgren

Donner Laboratory of Biophysics and Medical Physics, Lawrence Radiation Laboratory, University of California, Berkeley 4, California

Dialysis of three major lipoprotein fractions from human serum, Sf20-105, Sf0-20, and -S1.200-16, against low concentrations of cupric ion is associated with accelerated chemical alteration of polyunsaturated fatty acids and cholesterol. Less extensive changes in these acids were observed during dialysis against ion-free water. Reaction products of these chemical alterations were irreversibly retained on succinic acid-diethylene glycol polyester gas chromatographic columns. Present evidence suggests that these reaction products are relatively polar compounds, probably in the chemical class of lipoperoxides.

Submitted on December 14, 1960


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