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Journal of Lipid Research, Vol. 2, 215-222, July 1961
Copyright © 1961 by Lipid Research, Inc.

Wheat flour lipids: II. isolation and characterization of glycolipids of wheat flour and other plant sources

H. E. Carter , K. Ohno , S. Nojima , C. L. Tipton , and N. Z. Stanacev

Division of Biochemistry, Noyes Laboratory of Chemistry, University of Illinois, Urbana, Illinois

Methods involving solvent fractionation and silicic-acid column chromatography have been developed for isolating monogalactosyl- and digalactosylglycerol lipids and cerebrosides from the benzene-extracted lipids of wheat flour. A comparative study has been made of the composition of the lipid mixtures obtained from bleached and unbleached flours. The lipids from bleached flour were found to contain covalently bound chlorine, apparently resulting from reaction with chlorine, the bleaching agent. A preliminary study of the occurrence of galactosylglycerol lipids in other plant materials is reported.

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