Journal of Lipid Research, Vol. 2, 271-277, July 1961
Copyright © 1961 by Lipid Research, Inc.

Separation of dog serum lipoproteins by ultracentrifugation, dextran sulfate precipitation, and paper electrophoresis

Toshio Sakagami and D. B. Zilversmit

Division of Physiology, University of Tennessee, Memphis 3, Tennessee

The separation of dog serum lipoproteins by ultracentrifugation at a density of 1.063 is hindered by the failure of the high density fraction to accumulate in the bottom portion of the centrifuge tube. This phenomenon interferes with the quantitative recovery of pure low density lipoproteins. By paper electrophoresis, a lipid-containing protein with the mobility of a ßbeta;-globulin was detected in the lowest layer of the centrifuge tube. Comparison of the preparative ultracentrifuge technique with that of dextran sulfate precipitation of ßbeta;-lipoproteins revealed the suitability of the latter procedure for the quantitative separation of ßbeta;-lipoproteins. The dextran sulfate ßbeta;-lipoprotein precipitate was shown to be free from -lipoprotein by paper electrophoresis.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?