J. Lipid Res.
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Journal of Lipid Research, Vol. 2, 342-343, October 1961
Copyright © 1961 by Lipid Research, Inc.

Inhibition of cholesterol biogenesis by arsenite: preparation of labeled lanosterol

Mary L. Moller and T. T. Tchen

Department of Chemistry, Wayne State University, Detroit 2, Michigan

When mevalonic acid-2-C14 was incubated with rat liver homogenate with the usual cofactors plus 10-3 M arsenite, the formation of labeled cholesterol was inhibited and labeled lanosterol accumulated. This inhibitory effect of arsenite provides a convenient method of preparing labeled lanosterol in high yield (>10%) from commercially available C14-labeled mevalonic acid.

Submitted on March 29, 1961


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