Journal of Lipid Research, Vol. 2, 369-375, October 1961
Copyright © 1961 by Lipid Research, Inc.

The in vitro stimulation of lipogenesis and carbohydrate metabolism in liver by potassium ion and diphenylmethylacetyl-N-methylpiperazine

Frederick N. Minard and Raymond V. Davis

Department of Biochemistry, Research Division, Abbott Laboratories, North Chicago, Illinois

An experimental drug, diphenylmethylacetyl-N-methylpiperazine (A-11850), was found to stimulate the incorporation of acetate-1-C¹⁴, but not that of mevalonate-2-C¹⁴, into the fatty acid and nonsaponifiable fractions of rat liver slices. In addition, the drug increased about equally the oxidation of both glucose-1-C¹⁴ and glucose-6-C¹⁴ to C¹⁴O₂. The incubation of liver slices in a buffer containing a high level of potassium ion stimulated lipogenesis in the same manner as the drug; with potassium ion, however, the oxidation of glucose-1-C¹⁴ was increased to a much greater extent than that of glucose-6-C¹⁴. Potassium ion also stimulated glucose oxidation in liver homogenates, whereas A-11850 did not.

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