Journal of Lipid Research, Vol. 2, 383-388, October 1961
Copyright © 1961 by Lipid Research, Inc.

Metabolism of stearate-1-C¹⁴ in the isolated cow's udder

Monique Lauryssens , Roger Verbeke , and Georges Peeters

Department of Physiology, Veterinary College, University of Ghent, Ghent, Belgium

One-half of a lactating cow's udder was perfused with heparinized and oxygenated cow's blood at 38° for 150 minutes; 30 minutes after the onset of the perfusion, approximately 200 µc of stearate-1-C¹⁴ (albumin-bound) was added to the perfusing blood, together with inactive acetate and glucose. Almost all the blood's stearate-1-C¹⁴ was absorbed by the gland, and much of the added C¹⁴ was recovered from the glycerides of the udder tissue. Stearic acid and oleic acid of the glycerides showed significant specific activities. Negligible activities were detected in the shorter chain fatty acids of the glycerides, in citric acid, and in CO₂, indicating that stearic acid was broken down to only a very small extent.

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