Journal of Lipid Research, Vol 20, 116-124, Copyright © 1979 by Lipid Research, Inc.
Prostaglandin synthesis in rat adrenocortical cells
R Chanderbhan, VA Hodges, CR Treadwell and GV Vahouny
The biosynthesis of prostaglandins by isolated rat adrenocortical cells has
been studied by determinations of products formed during incubations with
labeled arachidonic acid and by radioimmunoassays. Analysis by thin-layer
chromatographic separation of silicic acid column fractions indicated that
PGE2, PGA2, (B2) and PGF2 alpha were the predominant prostaglandins formed
by rat adrenocortical cells. Approximately 75% of the incorporated isotope
was associated with the prostaglandins of the PGE pathway [PGE2 + PGA2
(B2)]. This was a consistent finding whether cells were incubated directly
with arachidonic acid or with cells prelabeled with the substrate prior to
study. ACTH did not affect the uptake or oxidation of [1-14C]-
arachidonate, but did significantly increase incorporation of labeled
substrate into [14C]prostaglandins. Of the ACTH-induced increase, 92% was
accounted for by an increase in prostaglandins of the E pathway. Studies
with prelabeled cells indicated that 77% of the prostaglandins synthesized
in both control and ACTH-stimulated adrenocortical cells was released into
the incubation medium during the 2-hr study. These had the same composition
[88% PGE2 + PGA2 (B2)] as did the intracellular prostaglandins. Analysis by
radioimmunoassays gave comparable data on the distribution of E- and F-type
prostaglandins in control cells and cells incubated with ACTH or dibutyryl
cyclic AMP. Thus, with these techniques, 88-92% of the increased
prostaglandin synthesis due to ACTH or cyclic AMP was produced by the PGE2
rather than the PGF2 alpha pathway.
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