J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol 20, 134-139, Copyright © 1979 by Lipid Research, Inc.

ARTICLES

Synthesis of [24, 25-3H]cholesterol: a new substrate for determining the rate of cholesterol side chain oxidation

CL Bentzen and K Brendel

A procedure for the synthesis of [24,25-3H]cholesterol from the nonradioactive precursor desmosterol is described. The intermediate, isodesmosterol, which was purified by column chromatography, was formed to protect the original double bond (delta 5-6) from hydrogenation. Tritium was introduced into the side chain by catalytic reduction of the double bond (delta 24-25) of the isodesmosterol in the presence of carrier-free tritium. After ring rearrangement of the iso-[24,25- 3H]cholesterol acetate, the acetate was hydrolyzed to form the free labeled cholesterol. Hepatic oxidation of the [24,25-3H]cholesterol side chain release tritium into water which freely equilibrates with cell and body water pools. Thus, the rate of 3H2O appearance corresponds to the rate of cholesterol side chain oxidation. Applications of this method to in vivo, isolated perfused liver, and isolated hepatocyte preparations of the rat are discussed.
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