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Journal of Lipid Research, Vol 20, 275-278, Copyright © 1979 by Lipid Research, Inc.


ARTICLES

An improved method for the covalent attachment of glycolipids to solid supports and macromolecules

WW Young Jr, RA Laine and S Hakomori

A simplified method is presented for the oxidation of the olefinic bond of the sphingosine moiety of glycosphingolipids to a carboxyl group. Coupling of such "glycolipid acids" to glass beads, agarose gels, proteins, and polyacrylic hydrazide polymers is described. Solid supports and macromolecules that have been derivatized in this fashion are useful reagents for a variety of studies in cell biology and immunology.
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M. Mylvaganam and C. A. Lingwood
A Convenient Oxidation of Natural Glycosphingolipids to Their "Ceramide Acids" for Neoglycoconjugation. BOVINE SERUM ALBUMIN-GLYCOSYLCERAMIDE ACID CONJUGATES AS INVESTIGATIVE PROBES FOR HIV gp120 COAT PROTEIN-GLYCOSPHINGOLIPID INTERACTIONS
J. Biol. Chem., July 16, 1999; 274(29): 20725 - 20732.
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