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Journal of Lipid Research, Vol 20, 408-412, Copyright © 1979 by Lipid Research, Inc.

ARTICLES

Phosphorus NMR analysis of phospholipids in detergents

E London and GW Feigenson

Various detergents can be used to dissolve phospholipids, resulting in very narrow 31PNMR resonances. The resonances are well resolved, allowing identification and quantitative analysis of phospholipids in a mixture. The chemical shift depends strongly on pH, reflecting changes in the state of ionization of the phospholipid headgroup moieties. Samples of phospholipids dissolved in aqueous detergents are conveniently prepared and give narrower 31P resonances than do phospholipids dissolved in organic solvents.
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