J. Lipid Res.
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Journal of Lipid Research, Vol 20, 413-416, Copyright © 1979 by Lipid Research, Inc.

ARTICLES

A comparison of delipidated sera used in studies of sterol synthesis by human mononuclear leukocytes

HR Slater and FW Robertson

Sterol synthesis in human mononuclear leukocytes is stimulated by delipidated serum. Synthesis in media containing serum delipidated by three different methods is compared. Significant differences between subjects are shown and the differences are maximized by measuring synthesis in serum delipidated by extraction with butanol-diisopropyl ether 40:60 and diethyl ether.
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Copyright © 1979 by the American Society for Biochemistry and Molecular Biology.