Journal of Lipid Research, Vol 20, 447-455, Copyright © 1979 by Lipid Research, Inc.
Enzyme-substrate interaction in lipid monolayers. II. Binding and activity of lipase in relation to enzyme and substrate concentration and to other factors
JP Barque and DG Dervichian
With the limited stirring procedure used in the present work, substrate and
enzyme together form a segregated and well-defined system on the surface.
The lipase molecules responsible for the lipolysis are only those that are
adsorbed on the glyceride monolayer. After a study of the stirring
procedure, two series of systematic experiments were done: a) the bulk
concentration of the enzyme was varied with different constant surface
concentrations of the substrate, and b) the surface concentration of the
substrate was varied with different constant bulk concentrations of the
enzyme. The influence of the surface concentration of substrate on a) the
rate of lipolysis, V,; b) the enzyme activity, a,; and c) the enzyme
adsorption, Ze, were each determined by a different procedure. The values
obtained verify the enzymic activity equation (a = V/Ze). The roles of
other factors (Ca2+ ions, and pH) which govern the adsorption of the enzyme
and its specific activity were also studied in preliminary experiments.
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