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Journal of Lipid Research, Vol 20, 561-563, Copyright © 1979 by Lipid Research, Inc.

ARTICLES

Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms

EK Ryu and M MacCoss

A simple modification of the Dittmer-Lester reagent is described that allow the detection of phospholipid derivatives at very low concentrations on silica gel and reversed-phase thin-layer plates. This modification, which involves the addition of acetic acid to the mixture, permits the observation of sharp blue spots on a white background. The specificity and sensitivity of the spray are discussed.
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