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Journal of Lipid Research, Vol. 20, 579-587, July 1979
Copyright © 1979 by Lipid Research, Inc.

Separation of molecular species of sphingomyelin by reversed-phase high-performance liquid chromatography

Firoze B. Jungalwala , Virginia Hayssen , Juana M. Pasquini , and Robert H. McCluer

Department of Biochemistry, Eunice Kennedy Shriver Center for Mental Retardation, Waltham, MA 02154 and Department of Neurology, Harvard Medical School, Boston, MA 02114

A convenient method for the separation of molecular species of sphingomyelin by reversed-phase high-performance liquid chromatography (HPLC) is described. Sphingomyelin species from bovine brain and sheep and pig erythrocytes were resolved into 10-12 separate peaks on a µ-BondaPak C₁₈ or Nucleosil-5-C₁₈ reversedphase column with methanol-5 mM potassium phosphate buffer, pH 7.4, 9:1 (v/v) as the solvent. Detection was at 203-205 nm. The sphingomyelin species were primarily resolved due to specific hydrophobic interaction of their fatty acid and sphingoid chains with the alkyl ligand of the stationary phase. The retention time of the sphingomyelin species increased progressively as the number of carbon atoms in the hydrophobic chains increased in the homologous series. The presence of one double bond in the molecule reduced the retention time significantly. Introduction of a second double bond in the fatty acid side chain did not reduce the retention time to the same extent as the first double bond. The presence of a trans double bond in the sphingoid moiety increased the retention time of sphingomyelin more than did a cis double bond in the fatty acid side chain. The differential hydrophobic interaction observed between the ligand of the stationary phase and different alkyl chains of the sphingomyelin species illustrates that reversed-phase HPLC technique can be conveniently used to study the extent of relative hydrophobicity of different types of alkyl chains.—Jungalwala, F. B., V. Hayssen, J. M. Pasquini, and R. H. McCluer. Separation of molecular species of sphingomyelin by reversed-phase high-performance liquid chromatography.

Supplementary key words fatty acid and sphingoid base composition • hydrophobic interaction • effective methylene number • bovine brain and pig and sheep erythrocyte sphingomyelins

Submitted on August 7, 1978
Accepted on December 18, 1978

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