Journal of Lipid Research, Vol 20, 599-606, Copyright © 1979 by Lipid Research, Inc.
Enzyme--substrate interaction in lipid monolayers. III. A study of the variation of the surface concentration with lipolysis
JP Barque and DG Dervichian
Monolayers of a diacylglycerol were submitted to the action of lipase,
keeping the area constant. The variation of lipase, keeping the area
constant. The variation of the surface concentration gamma of the substrate
with time was derived from the recorded reduction of the surface pressure
pi (the isotherm of the monolayer being previously established). The rate
-d gamma/dt was determined both as a function of the surface concentration
gamma of the substrate and as a function of the bulk concentration C of the
enzyme in the underlying solution. The rate depends on the quantity of
enzyme ze adsorbed on the monolayer and on the enzymatic specific activity
alpha of these adsorbed enzyme molecules. Both ze and alpha vary with
gamma. The two variations have been quantitatively dissociated. The curves
of ze and of alpha as functions of gamma coincide with those previously
established in the study of hydrolysis under constant surface pressure.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
