Journal of Lipid Research, Vol 20, 624-630, Copyright © 1979 by Lipid Research, Inc.
Effects of high-glucose and high-fat diets on concanavalin A binding to rat liver plasma membranes and on the amount and pattern of their glycoprotein carbohydrates
DS Henriquez, HM Tepperman and J Tepperman
Purified liver plasma membranes were prepared from rats fed a high-fat,
carbohydrate-free diet or a high-glucose, fat-free diet. Membranes from
rats fed the high-fat diet bound significantly less 125I-labeled
concanavalin A (Con A) than did those from rats fed the fat-free diets. The
magnitude of the binding difference increased with increasing
concentrations of Con A. Neither association nor dissociation rates of the
lectin-receptor complex was affected by diet. The extent of degradation of
Con A by liver plasma membrane preparations from rats fed either diet was
the same. Chemical analysis of delipidated liver plasma membrane showed
that membranes prepared from high-fat diet- adapted rats had significantly
lower values for all carbohydrate components measured with the exception of
galactose. The results indicate that, in liver cells, a change in plasma
membrane glycoproteins is part of the complex adaptation to altered diet
composition.
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