Journal of Lipid Research, Vol 20, 639-645, Copyright © 1979 by Lipid Research, Inc.

ARTICLES

A high-performance liquid chromatographic method for the determination of tocopherol in plasma and cellular elements of the blood

LJ Hatam and HJ Kayden

A rapid sensitive, and reproducible procedure is described for the analysis of alpha-tocopherol in blood cells and plasma using high- performance liquid chromatography and fluorometric detection. The cardinal feature for the increased sensitivity of this high-performance liquid chromatographic procedure is that the fluorometric analysis was carried out at a short excitation wavelength (205 nm) which increased the sensitivity of 20-fold over the usual excitation wavelength of 295 nm. Tocopherol levels can be measured in as little as 50 microliters of plasma and 200 microliters of erythrocytes. The tocopherol contentof plasma, red blood cells, platelets, polymorphonuclear leukocytes, and lymphocytes of normal subjects and subjects ingesting additional quantitites of vitamin E are reported. The values for the white cells are approximately 30 times higher than those of the red blood cells (polymorphonuclear leukocytes 4.47 +/- 0.62 micrograms/10(9), lymphocytes 3.89 +/- 0.85 micrograms/10(9), and erythrocytes 1.40 +/- 0.14 micrograms/10(10) cells). The tocopherol contents of the plasma and all the cellular elements of the blood were increased by oral feeding with vitamin E.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				V. O. Palmieri, I. Grattagliano, P. Portincasa, and G. Palasciano Systemic Oxidative Alterations Are Associated with Visceral Adiposity and Liver Steatosis in Patients with Metabolic Syndrome J. Nutr., December 1, 2006; 136(12): 3022 - 3026. [Abstract] [Full Text] [PDF]

				S. Colas, K. Maheo, F. Denis, C. Goupille, C. Hoinard, P. Champeroux, F. Tranquart, and P. Bougnoux Sensitization by dietary docosahexaenoic Acid of rat mammary carcinoma to anthracycline: a role for tumor vascularization. Clin. Cancer Res., October 1, 2006; 12(19): 5879 - 5886. [Abstract] [Full Text] [PDF]

				K. Anwar, H. J. Kayden, and M. M. Hussain Transport of vitamin E by differentiated Caco-2 cells J. Lipid Res., June 1, 2006; 47(6): 1261 - 1273. [Abstract] [Full Text] [PDF]

				M. F. Cury-Boaventura, R. Gorjao, T. M. de Lima, T. M. Piva, C. M. Peres, F. G. Soriano, and R. Curi Toxicity of a Soybean Oil Emulsion on Human Lymphocytes and Neutrophils JPEN J Parenter Enteral Nutr, March 1, 2006; 30(2): 115 - 123. [Abstract] [Full Text] [PDF]

				V. Scislowski, D. Bauchart, D. Gruffat, P. M. Laplaud, and D. Durand Effects of dietary n-6 or n-3 polyunsaturated fatty acids protected or not against ruminal hydrogenation on plasma lipids and their susceptibility to peroxidation in fattening steers J Anim Sci, September 1, 2005; 83(9): 2162 - 2174. [Abstract] [Full Text] [PDF]

				L.T. McGrath, B.M. McGleenon, S. Brennan, D. McColl, S. McILroy, and A.P. Passmore Increased oxidative stress in Alzheimer's disease as assessed with 4-hydroxynonenal but not malondialdehyde QJM, September 1, 2001; 94(9): 485 - 490. [Abstract] [Full Text] [PDF]

				S. Ching, D. C. Mahan, J. S. Ottobre, and K. Dabrowski Ascorbic Acid Synthesis in Fetal and Neonatal Pigs and in Pregnant and Postpartum Sows J. Nutr., July 1, 2001; 131(7): 1997 - 2001. [Abstract] [Full Text] [PDF]

				L. M. Maynard, G. A. Boissonneault, C. K. Chow, and G. G. Bruckner High Levels of Dietary Carnosine Are Associated with Increased Concentrations of Carnosine and Histidine in Rat Soleus Muscle J. Nutr., February 1, 2001; 131(2): 287 - 290. [Abstract] [Full Text]

				C.-Y. J. Lee and Fan Wan ; Vitamin E Supplementation Improves Cell-Mediated Immunity and Oxidative Stress of Asian Men and Women J. Nutr., December 1, 2000; 130(12): 2932 - 2937. [Abstract] [Full Text] [PDF]

				I. Grattagliano, G. Vendemiale, P. Caraceni, M. Domenicali, B. Nardo, A. Cavallari, F. Trevisani, M. Bernardi, and E. Altomare Starvation Impairs Antioxidant Defense in Fatty Livers of Rats Fed A Choline-Deficient Diet J. Nutr., September 1, 2000; 130(9): 2131 - 2136. [Abstract] [Full Text]

				W. H. Ibrahim, H. N. Bhagavan, R. K. Chopra, and C. K. Chow Dietary Coenzyme Q10 and Vitamin E Alter the Status of These Compounds in Rat Tissues and Mitochondria J. Nutr., September 1, 2000; 130(9): 2343 - 2348. [Abstract] [Full Text]

				K. J Lenton, H. Therriault, A. M Cantin, T. Fulop, H. Payette, and J R. Wagner Direct correlation of glutathione and ascorbate and their dependence on age and season in human lymphocytes Am. J. Clinical Nutrition, May 1, 2000; 71(5): 1194 - 1200. [Abstract] [Full Text] [PDF]

				J. W. Anderson, M. S. Gowri, J. Turner, L. Nichols, V. A. Diwadkar, C. K. Chow, and P. R. Oeltgen Antioxidant Supplementation Effects on Low-Density Lipoprotein Oxidation for Individuals with Type 2 Diabetes Mellitus J. Am. Coll. Nutr., October 1, 1999; 18(5): 451 - 461. [Abstract] [Full Text] [PDF]

				S. O. McGuire, D. W. Alexander, and K. L. Fritsche Fish Oil Source Differentially Affects Rat Immune Cell alpha -Tocopherol Concentration J. Nutr., July 1, 1997; 127(7): 1388 - 1394. [Abstract] [Full Text]

				W. Ibrahim, U.-S. Lee, C.-C. Yeh, J. Szabo, G. Bruckner, and C. K. Chow Oxidative Stress and Antioxidant Status in Mouse Liver: Effects of Dietary Lipid, Vitamin E and Iron J. Nutr., July 1, 1997; 127(7): 1401 - 1406. [Abstract] [Full Text]

				J. M. May, Z.-c. Qu, and J. D. Morrow Interaction of Ascorbate and alpha-Tocopherol in Resealed Human Erythrocyte Ghosts J. Biol. Chem., May 3, 1996; 271(18): 10577 - 10582. [Abstract] [Full Text] [PDF]

				M. Fariss, G. Pascoe, and D. Reed Vitamin E reversal of the effect of extracellular calcium on chemically induced toxicity in hepatocytes Science, February 15, 1985; 227(4688): 751 - 754. [Abstract] [PDF]