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Journal of Lipid Research, Vol. 20, 646-653, July 1979
Copyright © 1979 by Lipid Research, Inc.

Intestinal absorption and lymphatic transport of cholesterol and ßbeta;-sitostanol in the rat

Aslam S. Hassan and Alfred J. Rampone

Department of Physiology, School of Medicine, University of Oregon Health Sciences Center, Portland, OR 97201

The intestinal absorption of cholesterol and ßbeta;-sitostanol (the saturated analogue of ßbeta;-sitosterol) were measured and their absorptions compared in the presence and absence of cholestyramine. After test meals containing [³H]cholesterol and [¹⁴C]ßbeta;-sitostanol without added cholestyramine, 4-day fecal collections yielded an average of 51% of the fed cholesterol and 83% of the fed ßbeta;-sitostanol. In separate lymph transport studies without cholestyramine, 36% of the fed cholesterol was recovered in lymph in 24 hours compared to only 2% of the fed ßbeta;-sitostanol. Thus, while total recoveries of the two labeled compounds in feces plus lymph were nearly identical (51% + 36% = 87% for cholesterol and 83% + 2% = 85% for ßbeta;-sitostanol) their distribution in the two compartments was markedly different, reflecting the relative nonabsorbability of ßbeta;-sitostanol. Adding cholestyramine to the test meal caused fecal excretion of cholesterol to increase to 73%, independent of the dose of cholestyramine used. Cholestyramine had no effect on the fecal excretion of ßbeta;-sitostanol (average excretion after cholestyramine, 85%). The relative non-absorbability of ßbeta;-sitostanol compared to cholesterol is clearly evident in this study and leads us to suggest its possible use as a lipid-soluble, nonabsorbable reference compound for measurement of the absorption of cholesterol and other lipids. Further data are presented to justify its use for this purpose.—Hassan, A. S., and A. J. Rampone. Intestinal absorption and lymphatic transport of cholesterol and ßbeta;-sitostanol in the rat.

Supplementary key words plant sterols • cholestyramine • thoracic duct • fecal excretion of sterols

Submitted on September 18, 1978
Accepted on December 29, 1978

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