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Journal of Lipid Research, Vol 20, 681-685, Copyright © 1979 by Lipid Research, Inc.

ARTICLES

Sterol synthesis. High-pressure liquid chromatography of C27 sterol precursors of cholesterol

JR Thowsen and GJ Schroepfer Jr

Conditions have been described which permit the rapid (approximately 3.5 hr) column chromatographic separation of the acetate derivatives of a number of C27 sterol precursors of cholesterol differing only in the number and position of double bonds in the sterol nucleus. On columns containing muPorasil with hexane-benzene 9:1 as the eluting solvent, acetate derivatives of the delta5, delta8(14), delta8, delta7, delta8,7, delta 8,14, and delta7,14 sterols were separated.
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