Increased cellular proliferation in adipose tissue of adult rats fed a high-fat diet

BJ Klyde and J Hirsch

The feeding of a high-fat diet to adult rats was shown to increase the incorporation of [3H]thymidine into DNA of the adipocyte and stromal fractions. After only 2 days on a high-fat diet there was a marked increase in the incorporation of label. When a 2-week period was interposed between [3H]thymidine administration and determination of DNA specific activity, the greatest increase in incorporation of label was found after 1 week on the diet, when incorporation increased 6-fold or more in both adipocytes and stroma and subsequently decreased to stabilize at a level two or three times that of chow-fed rats in the adipocyte fraction. Rats labeled when young and later placed on a high-fat diet showed a decrease in DNA specific activity in both adipocytes and stroma, confirming that cellular proliferation had occurred in both fractions. The specific activities of both stromal and adipocyte DNA were very similar at all time points studied. An attempt to increase the difference in specific activities by waiting many weeks after [3H]thymidine injection before isolating DNA was not successful. This may be because the total amount of DNA in the stromal and adipocyte fractions increases in parallel on the diet. The significance of these findings in terms of the normal turnover of adipose tissue DNA and the responsiveness to diet is discussed.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. R. Bowers, W. T. L. Festuccia, C. K. Song, H. Shi, R. H. Migliorini, and T. J. Bartness Sympathetic innervation of white adipose tissue and its regulation of fat cell number Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2004; 286(6): R1167 - R1175. [Abstract] [Full Text] [PDF]

				K.-H. Kim, L. Zhao, Y. Moon, C. Kang, and H. S. Sul Dominant inhibitory adipocyte-specific secretory factor (ADSF)/resistin enhances adipogenesis and improves insulin sensitivity PNAS, April 27, 2004; 101(17): 6780 - 6785. [Abstract] [Full Text] [PDF]

				Y.-H. Yu and H. Zhu Chronological changes in metabolism and functions of cultured adipocytes: a hypothesis for cell aging in mature adipocytes Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E402 - E410. [Abstract] [Full Text]

				R. K. Petersen, C. Jorgensen, A. C. Rustan, L. Froyland, K. Muller-Decker, G. Furstenberger, R. K. Berge, K. Kristiansen, and L. Madsen Arachidonic acid-dependent inhibition of adipocyte differentiation requires PKA activity and is associated with sustained expression of cyclooxygenases J. Lipid Res., December 1, 2003; 44(12): 2320 - 2330. [Abstract] [Full Text] [PDF]

				D. L. Crandall, D. E. Busler, B. McHendry-Rinde, T. M. Groeling, and J. G. Kral Autocrine Regulation of Human Preadipocyte Migration by Plasminogen Activator Inhibitor-1 J. Clin. Endocrinol. Metab., July 1, 2000; 85(7): 2609 - 2614. [Abstract] [Full Text]

				C. Bastie, D. Holst, D. Gaillard, C. Jehl-Pietri, and P. A. Grimaldi Expression of Peroxisome Proliferator-activated Receptor PPARdelta Promotes Induction of PPARgamma and Adipocyte Differentiation in 3T3C2 Fibroblasts J. Biol. Chem., July 30, 1999; 274(31): 21920 - 21925. [Abstract] [Full Text] [PDF]

				B. G. Marques, D. B. Hausman, and R. J. Martin Association of fat cell size and paracrine growth factors in development of hyperplastic obesity Am J Physiol Regulatory Integrative Comp Physiol, December 1, 1998; 275(6): R1898 - R1908. [Abstract] [Full Text] [PDF]

				S. Mandrup, R. V. Sorensen, T. Helledie, J. Nohr, T. Baldursson, C. Gram, J. Knudsen, and K. Kristiansen Inhibition of 3T3-L1 Adipocyte Differentiation by Expression of Acyl-CoA-binding Protein Antisense RNA J. Biol. Chem., September 11, 1998; 273(37): 23897 - 23903. [Abstract] [Full Text] [PDF]

				M. Okuno, K. Kajiwara, S. Imai, T. Kobayashi, N. Honma, T. Maki, K. Suruga, T. Goda, S. Takase, Y. Muto, et al. Perilla Oil Prevents the Excessive Growth of Visceral Adipose Tissue in Rats by Down-Regulating Adipocyte Differentiation J. Nutr., September 1, 1997; 127(9): 1752 - 1757. [Abstract] [Full Text]

				C. Bastie, S. Luquet, D. Holst, C. Jehl-Pietri, and P. A. Grimaldi Alterations of Peroxisome Proliferator-activated Receptor delta Activity Affect Fatty Acid-controlled Adipose Differentiation J. Biol. Chem., December 1, 2000; 275(49): 38768 - 38773. [Abstract] [Full Text] [PDF]