Journal of Lipid Research, Vol 20, 817-824, Copyright © 1979 by Lipid Research, Inc.
Interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol
A Jonas
The interaction of sonicated, small vesicles of egg phosphatidylcholine and
cholesterol (2:1, mol/mol) with bovine high density serum lipoproteins was
examined in terms of lipid transfer between both types of particles and the
resulting changes in lipoprotein structure. Saturation of high density
lipoprotein preparations with vesicle lipids gave final lipoprotein
particles with essentially unchanged protein content and composition,
unchanged cholesterylester and nonpolar lipid content, but with markedly
increased phospholipid content (59% increas by weight) and moderately
increased cholesterol content (20% increase by weight). The lipoproteins
enriched in lipid were relatively uniform, spherical particles, 110 +/- 3.6
A in diameter (6 A larger than the original lipoproteins); they had a
markedly decreased intrinsic protein fluorescence, a red-shifted
fluorescence wavelength maximum, and more fluid lipid domains. These
results indicate that the direct addition of excess lipids from membranes
or other lipoproteins is a possible mechanism for lipid transfer to high
density lipoproteins. Also they suggest a structural flexibility of high
density lipoproteins that allows the addition of significant amounts of
surface components.
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