J. Lipid Res.
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Journal of Lipid Research, Vol 20, 912-914, Copyright © 1979 by Lipid Research, Inc.

ARTICLES

Measurement of arachidonic acid in the plasma by gas-liquid chromatography-flame ionization using dihomo-gamma-linolenic acid as an internal standard

JG Gerber, JS Barnes and AS Nies

A gas-liquid chromatography-flame ionization method is described for measuring arachidonic acid in plasma using dihomo-gamma-linolenic acid as an internal standard. We found this technique to -e reproducible, and quicker and superior to previously described techniques because of the similar physico-chemical properties of the unsaturated fatty acid internal standard and arachidonic acid. In addition, we observed that the use of the saturated fatty acid, n-tricosanoic acid, was unsatisfactory as an internal standard because of its poor extractability from plasma as compared to arachidonic acid.
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Copyright © 1979 by the American Society for Biochemistry and Molecular Biology.