Journal of Lipid Research, Vol 20, 1036-1040, Copyright © 1979 by Lipid Research, Inc.
Characterization of the multiple-chamber perifused fat cell system
DO Allen, KJ Long and JT Majors
A multiple-chamber perifused fat cell system is described. Six chambers
containing fat cells were perifused in parallel with buffer. Perifusate was
collected for assay of glycerol as an index of lipolytic rates and cells in
each chamber can be taken for analysis of biochemical intermediates. The
system is so designed that drugs can be infused into the buffer and equally
distributed in each chamber or can be individually infused into the buffer
to one chamber, allowing for six different conditions to be tested in the
same population of fat cells. The time and distribution characteristics of
infused material are described. Time relationships are described for
isoproterenol and glycerol release and for cyclic AMP levels in the fat
cells, and the dose-response relationship between isoproterenol and
glycerol release is shown.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
