Journal of Lipid Research, Vol 21, 110-117, Copyright © 1980 by Lipid Research, Inc.
Biliary bile acids, bile alcohols, and sterols of Alligator mississippiensis
GS Tint, B Dayal, AK Batta, S Shefer, T Joanen, L McNease and G Salen
Bile from Alligator mississippiensis was found to contain a mixture of more
than twenty bile acids, bile alcohols, and neutral sterols. Bile acids and
bile alcohols were purified by reversed-phase high performance liquid
chromatography and thin-layer chromatography. Concentrations were measured
by gas-liquid chromatography on 1% HiEFF- 8BP and identifications were made
by mass spectrometry. The major neutral sterols consisted of 98%
cholesterol and 0.8% cholestanol. Bile acids recovered from the acidic
fraction were 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestanoic
acid (61%), 3 alpha, 7 alpha- dihydroxy-5 beta-cholestanoic acid (9%), 3
alpha, 7 alpha, 12 alpha- trihydroxy-5 alpha-cholestanoic acid (8%), and
3-oxo-7 alpha, 12 alpha- dihydroxy-5 beta-cholestanoic acid (10%). Other
C27 bile acids identified were: 3 alpha, 12 alpha-dihydroxy-5
beta-cholestanoic acid, 7-oxo-3 alpha, 12 alpha-dihydroxy-5
beta-cholestanoic acid, and 3-oxo-7 alpha, 12 alpha-dihydroxy-5
alpha-cholestanoic acid. Small quantities of C24 cholic, 5 alpha-cholic,
chenodeoxycholic, and ursodeoxycholic acids were also detected, as were
trace amounts of the C27 bile alcohols 5 alpha-cholestane-3 alpha, 7 alpha,
12 alpha, 26-tetrol, and 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha,
25-tetrol. These results suggest that the alligator is acpable of
synthesizing both the 5 alpha and 5 beta-isomers of the C27 bile acids. The
small amounts of the C24 bile acids present might originate either from C27
bile acid or bile alcohol precursors or from exogenous sources.
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