Journal of Lipid Research, Vol 21, 123-130, Copyright © 1980 by Lipid Research, Inc.

ARTICLES

Quantification of low density lipoprotein binding and cholesterol accumulation by single human fibroblasts using fluorescence microscopy

HS Kruth and M Vaughan

Using fluorescence microscopy, we have quantified low density lipoprotein (LDL) binding by indirect immunofluorescence and cellular cholesterol with the fluorescent sterol-binding polyene, filipin, in individual cultured human fibroblasts from normal subjects and from patients with heterozygous and homozygous familial hypercholesterolemia. LDL binding by fibroblasts from heterozygous patients was about 40% of that of the normal cells, and cholesterol accumulation upon incubation with LDL was decreased to a similar degree. Most fibroblasts from homozygous patients bound no detectable LDL and only rare cells demonstrated any accumulation of cholesterol after incubation with LDL.
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