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Journal of Lipid Research, Vol 21, 309-315, Copyright © 1980 by Lipid Research, Inc.
ARTICLES |
BA Hanson and RL Lester
By use of fungi grown in the presence of [3H]-inositol and [14C]choline, we have explored methods for the quantitative extraction of inositol-containing phospholipids and phosphatidylcholine. Slightly alkaline mixtures of both ethanol-water and ethanol-diethylether-water at elevated temperatures were shown to effectively extract these lipids from intact Saccharomyces cerevisiae and Neurospora crassa. Some previously published procedures fail to completely extract to very polar phosphoinositol-containing sphingolipids of these organisms. Trichloroacetic acid can be used with caution in killing cells prior to extraction; lipid destruction can occur at elevated concentrations and temperatures. Complete extraction of these very polar lipids with polar solvents also results in an extract containing significant amounts of non-lipids.
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