J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol 21, 449-454, Copyright © 1980 by Lipid Research, Inc.

ARTICLES

Synthesis of molecular species of glycerophospholipids from diglyceride- labeled brain microsomes

R Roberti, L Binaglia and G Porcellati

Selectivity of CDP-choline:diacylglycerol choline phosphotransferase and CDP-ethanolamine:diacylglycerol ethanolamine phosphotransferase for molecular species of diglyceride has been studied in rat brain microsomes in vitro. Diglyceride-labeled microsomes were prepared by incubation with labeled sn-glycerol-3-phosphate; the microsomes were then incubated with CDP-choline or CDP-ethanolamine for different time intervals. Experimental data extrapolated to zero-time incubation were taken into account for evaluating species specificity. A small selectivity for diglyceride species has been demonstrated for the choline phosphotransferase, but the ethanolamine phosphotransferase was found to convert hexaenoic diglyceride into phospholipid at the highest rate.
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Copyright © 1980 by the American Society for Biochemistry and Molecular Biology.