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Journal of Lipid Research, Vol 21, 473-477, Copyright © 1980 by Lipid Research, Inc.

ARTICLES

An improved technique for separation of neutral glycosphingolipids by high-performance liquid chromatography

A Suzuki, SK Kundu and DM Marcus

We have developed a high-performance liquid chromatographic (HPLC) procedure for separation of O-acetyl-N-p-nitrobenzoyl derivatives of six neutral glycosphingolipids: glucosylceramide, lactosylceramide, globotriaosylceramide, lactotriaosylceramide, globotetraosylceramide, and neolactotetraosylceramide. The recoveries of glucosylceramide and globotetraosylceramide for the derivatization procedure and HPLC analysis were approximately 75%, and one nanomole of glycolipid could be detected. The procedure was used for analysis of human erythrocyte neutral glycolipids.
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