Journal of Lipid Research, Vol 21, 477-480, Copyright © 1980 by Lipid Research, Inc.
Evaluation of the 3 alpha-hydroxysteroid dehydrogenase assay for ursodeoxycholic acid, and 7 oxo- and 12 oxo- bile acids
LJ Haeffner, SJ Gordon, JS Magen and OD Kowlessar
3 alpha-Hydroxysteroid dehydrogenase (SDH) quantifies ursodeoxycholic acid,
3 alpha,12 alpha-diol-7-one-5 beta-cholanoic acid, 3 alpha-ol-7- one-5
beta-cholanoic acid, and 3 alpha,7 alpha-diol-12-one-5 beta- cholanoic acid
in a manner similar to the more commonly measured bile acids, exemplified
by taurocholic acid. The type of oxygen function at the 7 or 12 position
and its orientation at the 7 position has no effect on the rate of reaction
of the enzyme. Discrepancies in the glycine/taurine ratios of patient
intestinal aspirates containing ursodeoxycholic acid and oxo bile acids,
obtained by SDH and gas-liquid chromatographic methods are not a result of
the enzyme assay procedure.
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