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Journal of Lipid Research, Vol 21, 480-484, Copyright © 1980 by Lipid Research, Inc.

ARTICLES

Loss of tritium from coprostanone derived from [1,2(n)-3H]cholesterol or [7(n)-3H]cholesterol

GE Mott and EM Jackson

After oral administration of a mixture of [1,2(n)-3H]cholesterol and [4- 14C]cholesterol to a baboon, fecal coprostanone had a 46% lower 3H/14C ratio than the dose administered. Loss of 3H by enolization of the 3- ketone could account for the decrease in 3H/14C. If [7(n)- 3H]cholesterol was administered instead of [1,2(n)-3H]cholesterol a 23% loss of 3H from coprostanone was found. Procedures requiring measurement of 3H-coprostanone derived from [1,2(n)-3H]- or [7(n)- 3H]cholesterol could be seriously in error unless an appropriate correction for loss of 3H is made.
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