Journal of Lipid Research, Vol 21, 491-495, Copyright © 1980 by Lipid Research, Inc.

ARTICLES

A rapid and simple method for measurement of total protein in very low density lipoproteins by the Lowry assay

ML Kashyap, BA Hynd and K Robinson

When total protein in very low density lipoprotein samples is measured by the method of Lowry et al. (1951. J. Biol. Chem 193:265-275), turbidity remains in the final color reaction. Addition of 0.1 ml of 2.5% (v/v) solution of Triton X-100 removes this turbidity immediately and effectively. This simple modification is faster, less cumbersome, and more economical than removing turbidity with ethyl ether or chloroform. Addition of 0.1 ml of 2.5% (w/v) sodium dodecyl sulfate to the final reaction mixture is also effective in removing turbidity and can be used as an alternative method.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Adiels, C. Packard, M. J. Caslake, P. Stewart, A. Soro, J. Westerbacka, B. Wennberg, S.-O. Olofsson, M.-R. Taskinen, and J. Boren A new combined multicompartmental model for apolipoprotein B-100 and triglyceride metabolism in VLDL subfractions J. Lipid Res., January 1, 2005; 46(1): 58 - 67. [Abstract] [Full Text] [PDF]

				R. Malmstrom, C. J. Packard, T. D. G. Watson, S. Rannikko, M. Caslake, D. Bedford, P. Stewart, H. Yki-Jarvinen, J. Shepherd, and M.-R. Taskinen Metabolic Basis of Hypotriglyceridemic Effects of Insulin in Normal Men Arterioscler. Thromb. Vasc. Biol., July 1, 1997; 17(7): 1454 - 1464. [Abstract] [Full Text]