J. Lipid Res. Please sign the JLR Guestbook
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Feighner, S. D.
Right arrow Articles by Hylemon, P. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Feighner, S. D.
Right arrow Articles by Hylemon, P. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol 21, 585-593, Copyright © 1980 by Lipid Research, Inc.

ARTICLES

Characterization of a corticosteroid 21-dehydroxylase from the intestinal anaerobic bacterium, Eubacterium lentum

SD Feighner and PB Hylemon

An oxygen-sensitive corticosteroid 21-dehydroxylase has been characterized in cell extracts of Eubacterium lentum. The enzyme was highly specific for corticosteroids containing and alpha-ketol structure and required FMNH2 or reduced benzyl viologen for activity. The enzyme used deoxycorticosterone, deoxycortisol, dehydrocorticosterone, and corticosterone as substrates. Substrate saturation kinetics using [3H]corticosterone yielded an apparent Km of 7.35 microM and a Vmax of 15.4 nmol (11 beta-[3H]hydroxyprogesterone) formed per hr x mg protein-1. 21-Dehydroxylase activity was inhibited by both water-soluble and lipophilic metal ion chelators. NADH: flavin oxidoreductase and 21-dehydroxylase activities were separated by anaerobic DEAE-cellulose and Sepharose 6B chromatography. 21- Dehydroxylase had a relative weight of 582,000 as determined by Sepharose 6B chromatography. There was a 7-fold increase in the rate of 21-dehydroxylation of [3H]deoxycorticosterone in whole cell suspensions of E. lentum sparged with H2 as compared to argon gas.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 1980 by the American Society for Biochemistry and Molecular Biology.