Journal of Lipid Research, Vol 21, 625-634, Copyright © 1980 by Lipid Research, Inc.

ARTICLES

Characterization of apolipoprotein E-rich high density lipoproteins in familial lecithin:cholesterol acyltransferase deficiency

CD Mitchell, WC King, KR Applegate, T Forte, JA Glomset, KR Norum and E Gjone

We have isolated and charachterized a subfraction of high density lipoproteins, rich in apolipoprotein E, from the plasma of patients afflicted with familial lecithin:cholesterol acyltransferase deficiency. Prepared by successive ultracentrifugal flotation, affinity chromatography on heparin-agarose, and affinity chromatography on conconavalin A-agarose, the subfraction contained disc-shaped lipoproteins that measured 14--40 nm in diameter and 4.4--4.5 nm in thickness. The major components were apolipoprotein E, phosphatidylcholine, and unesterified cholesterol, though other apolipoproteins and lipids were present in small amounts. A second subfraction of high density lipoproteins, isolated during the chromatography, contained apolipoproteins A-I and A-II, but no apolipoprotein E. This subfraction included disc-shaped lipoproteins, 13--24 nm in diameter, as well as small round particles, 5.7 nm in diameter. Both subfractions contained similar proportions of total protein relative to lipid, similar amounts of unesterified cholesterol relative to phosphatidylcholine, and a similar distribution of phosphatidylcholine fatty acid.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. A. Cox, S. Birnbaum, M. C. Mahaney, D. L. Rainwater, J. T. Williams, and J. L. VandeBerg Identification of Promoter Variants in Baboon Endothelial Lipase That Regulate High-Density Lipoprotein Cholesterol Levels Circulation, September 4, 2007; 116(10): 1185 - 1195. [Abstract] [Full Text] [PDF]

				K.-A. Rye, R. Bright, M. Psaltis, and P. J. Barter Regulation of reconstituted high density lipoprotein structure and remodeling by apolipoprotein E J. Lipid Res., May 1, 2006; 47(5): 1025 - 1036. [Abstract] [Full Text] [PDF]

				B. Zhang, P. Fan, E. Shimoji, H. Xu, K. Takeuchi, C. Bian, and K. Saku Inhibition of Cholesteryl Ester Transfer Protein Activity by JTT-705 Increases Apolipoprotein E-Containing High-Density Lipoprotein and Favorably Affects the Function and Enzyme Composition of High-Density Lipoprotein in Rabbits Arterioscler. Thromb. Vasc. Biol., October 1, 2004; 24(10): 1910 - 1915. [Abstract] [Full Text] [PDF]

				D. S. Ng, O. L. Francone, T. M. Forte, J. Zhang, M. Haghpassand, and E. M. Rubin Disruption of the Murine Lecithin:Cholesterol Acyltransferase Gene Causes Impairment of Adrenal Lipid Delivery and Up-regulation of Scavenger Receptor Class B Type I J. Biol. Chem., June 20, 1997; 272(25): 15777 - 15781. [Abstract] [Full Text] [PDF]