HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Miller, J. P. Articles by Gotto, A. M. Search for Related Content PubMed PubMed Citation Articles by Miller, J. P. Articles by Gotto, A. M., Jr Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 21, 775-780, Copyright © 1980 by Lipid Research, Inc.

ARTICLES

The measurement of apolipoprotein A-I in human plasma by electroimmunoassay

JP Miller, SJ Mao, JR Patsch and AM Gotto Jr

A variable proportion of the total apolipoprotein A-I (apo A-I) present in plasma or high density lipoproteins (HDL) is normally detectable by immunochemical methods. This has been attributed to masking of some of the immunoreactive sites of apo A-I by lipid in the intact HDL particle. This difficulty has been circumvented by heating or delipidation. We find that exposure of plasma to concentrations of urea greater than about 7.0 M in the barbital buffer used to dilute plasma samples for estimation by electroimmunoassay enables the complete detection of Apo A-I, as judged by comparison with samples delipidated with tetramethylurea. The need for time-consuming heating or delipidation is avoided.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. A. C. McPherson, I. S. Young, B. McKibben, and J. McEneny High density lipoprotein subfractions: isolation, composition, and their duplicitous role in oxidation J. Lipid Res., January 1, 2007; 48(1): 86 - 95. [Abstract] [Full Text] [PDF]

				K. Miwa, N. Yoshida, K. Nakagawa, and H. Inoue High-density lipoprotein particles are large in patients with variant angina Cardiovasc Res, March 1, 1998; 37(3): 729 - 737. [Abstract] [Full Text] [PDF]

				P. S. Bachorik, K. L. Lovejoy, M. D. Carroll, and C. L. Johnson Apolipoprotein B and AI distributions in the United States, 1988–1991: results of the National Health and Nutrition Examination Survey III (NHANES III) Clin. Chem., December 1, 1997; 43(12): 2364 - 2378. [Abstract] [Full Text] [PDF]