Journal of Lipid Research, Vol 21, 780-783, Copyright © 1980 by Lipid Research, Inc.
Routine assay for determination of alpha-tocopherol in liver
AW Kormann
A rapid and accurate procedure for determination of alpha-tocopherol in
liver has been developed. Extracts of saponified liver homogenates were
purified by a simple and efficient chromatography step on Kieselgel.
alpha-Tocopherol content of the purified extracts was determined by an
automated gas--liquid chromatographic assay of the trimethylsilyl
derivatives. Mean recovery for alpha-tocopherol added in vitro was 95.4 +/-
1.2% (SEM, 149 estimations). The lower assay limit was ca. 0.5 micrograms
alpha-tocopherol/g liver. An experienced analyst was able to process at
least 12 chick, rat, or geuinea pig livers per day.