Journal of Lipid Research, Vol 21, 879-887, Copyright © 1980 by Lipid Research, Inc.
Characterization of glycosphingolipids by direct inlet chemical ionization mass spectrometry
T Ariga, T Murata, M Oshima, M Maezawa and T Miyatake
Permethylated derivatives of cerebrosides and ceramide di-, tri-, tetra- ,
and penta-hexosides were analyzed by the direct inlet ammonia chemical
ionization (CI) mass spectrometry. In the CI mass spectra, the fragment
ions produced by the loss of methanol from the protonated molecular ion
were observed in all of the glycosphingolipids. Other fragment ions due to
the cleavage of glycosidic moiety were major ones under the CI conditions.
These ions provide information on the molecular species of
glycosphingolipids and the sugar sequence of their oligosaccharides.
Glycosphingolipids with hydroxy fatty acids could also be differentiated
from those with nonhydroxy fatty acids by comparing the intensities of
characteristic fragment ions. The CI method should be particularly useful
in structural studies of glycosphingolipids from natural sources.
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