Journal of Lipid Research, Vol 21, 1004-1014, Copyright © 1980 by Lipid Research, Inc.
Quantification of surfactant phospholipids in the dog lung
SF Ryan, SA Hashim, G Cernansky, CR Barrett Jr, AL Bell Jr and DF Liau
We quantified total phospholipid (PL), total and disaturated
phosphatidylcholine (PC and DSPC), phosphatidylglycerol (PG), and total
protein in alveolar washings and lung tissue in 22 dog lungs. Quantitative
recovery of alveolar material and assessment of its possible contamination
by blood lipids were important determinants of methodology. To remove
blood, the vessels of half the lungs were perfused with a fluorocarbon
emulsion before lavage. The volume of blood removed by perfusion and the
quantity and fatty acid patterns of its whole blood and plasma PL and PC
were determined. Washings of unperfused lungs contained means of 21% more
PL and 24% more PC than those of perfused lungs. Although this excess could
be accounted for by the PL and PC in pulmonary blood, the hemoglobin and
total protein content of washings and their PC fatty acid patterns
indicated that blood lipids were not a major source of the excess lipid in
washings of unperfused lungs. Using more recent morphometric estimates
rather than the indirect ones previously used by others, the quantity of
alveolar DSPC (1 mg/g lung) is calculated to be 1.8 times the amount
necessary to form a packed monolayer on the internal surface of the lung at
functional residual capacity.
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